產品介紹

Click-&-Go^® Click Chemistry Capture 試劑盒為具有疊氮化物或炔烴標記的蛋白質以及相應的點擊樹脂的研究人員提供了共價捕獲標記蛋白質的方法。一旦通過銅催化的點擊化學共價連接到樹脂上，珠子就可以以的嚴格性洗滌，幾乎消除了任何非特異性結合的蛋白質，從而產生高度富集的目的分子群體。該試劑盒包含特殊配方的組分，既能催化點擊標記反應，又能防止與炔烴或疊氮化物改性樹脂的非特異性結合。其中對應的富集介質，試劑盒里面不包括需要單獨訂購。使用者根據(jù)自己的實驗設計，蛋白標記的基團選擇反應對應的瓊脂糖。炔基標記的蛋白，選擇疊氮脂糖樹脂（貨號：1038-2），疊氮標記的蛋白，選擇炔基瓊脂糖樹脂（貨號：1032-2）。

Product introduction

Click-&-Go^® Click Chemistry Capture Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click resin with the means to covalently capture labeled proteins. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. The kit contains specially formulated components to both catalyze the click labeling reaction and prevent non-specific binding to alkyne- or azide-modified resin*.    * enrichment media is not provided.

Click-&-Go^®Click Chemistry Capture Kit provides researchers who have proteins labeled with an azide or alkyne and the corresponding click resin with the means to covalently capture labeled proteins. Once covalently attached to the resin via copper catalyzed click chemistry, beads can be washed with highest stringency virtually eliminating any non-specifically bound proteins to yield a highly enriched population of nascent molecules. The kit contains specially formulated components to both catalyze the click labeling reaction and prevent non-specific binding to alkyne- or azide-modified resin.

Captured proteins can be protease digested to yield a highly pure peptide pool that is ideal for mass spectrometry (e.g., LC MS/MS) based analysis, or release from beads under appropriate cleavage conditions for downstream analysis.

Click-&-Go^® Click Chemistry Capture Kit is comparable non-cleavable agarose azide/alkyne, Dde, PC, and disulfide agarose resin.

The biotin-free enrichment improves signal to noise by eliminating nonspecific binding and increasing selectivity, thus improving detection of low abundant proteins. This approach is fully compatible with widely used MS techniques including iTRAC and ICAT.

The biotin-free, covalent capture approach is superior to biotin or lectin based enrichment approaches, and ideal for proteomics, biomarker discovery, posttranslational modification (PTM) analysis, and more.

方法對比

Schematic representation of pull-down workflows for biotin-streptavidin and Click-&-Go^® enrichment Protocols