Carbapenem-resistant Klebsiella pneumoniae (CRKP)感染小鼠模型，全肺臟流式檢測分群圈門策略，可以參考下圖。Gating strategy for determining the myeloid and lymphoid subsets in the whole lungs of control and Trpv1DTA mice after 48h of clodronate liposome (CLL) administration. PBS-liposome (PBL) was used for control group of mice. CLL or PBL (70 μL/mouse) was inoculated via intratracheal route at 48 hours before the immune cell type analyses. 單核巨噬清除訂購信息見后。

氯膦酸鹽脂質體清除肺臟巨噬細胞全肺流式檢測解決方案

(A) Gating strategy for determining the myeloid and lymphoid subsets in the whole lungs of control and Trpv1DTA mice after 48h of clodronate liposome (CLL) administration. PBS-liposome (PBL) was used for control group of mice. CLL or PBL (70 μL/mouse) was inoculated via intratracheal route at 48 hours before the immune cell type analyses. Interstitial macrophages (IMs, CD45+Siglec-F–CD11b+Ly6G– F4/80+ cells), neutrophils (CD45+Siglec-F–CD11b+Ly6G+ cells), Ly6Chi monocytes (CD45+Siglec-F–CD11b+Ly6G– F4/80– Ly6Chi cells), Ly6Clo monocytes (CD45+Siglec-F–CD11b+Ly6G–F4/80–Ly6Clo cells), CD11b+ DC (CD45+Siglec-F–CD11b+Ly6G– F4/80–Ly6C– CD11c+ MHC-II+cells), CD11b– DCs (CD45+Siglec-F–CD11b– CD3– B220– NK1.1– CD11c+ MHC-II+cells), B cells (CD45+Siglec-F–CD11b– CD3–NK1.1–B220+cells), CD4+ T cells (CD45+Siglec-F–CD11b– CD3+ CD8–CD4+ cells), CD8+ T cells (CD45+Siglec-F–CD11b– CD3+ CD4–CD8+ cells) and CD4– CD8– T cells (CD45+Siglec-F–CD11b– CD3+ CD4–CD8– cells).

(B) Absolute numbers of myeloid and lymphoid subsets in the whole lungs of CLL- or PBL-treated control (n=3-4) and Trpv1DTA (n=3) mice after 48h of treatment.

(C) The BALF number of Ly6Chi monocytes, neutrophils, and AMs in CLL- and PBL-treated control (n=4) and Trpv1DTA mice (n=3-4) after 24h of CRKP infection. Statistical analysis: Mann Whitney test (B, left), unpaired ttests (B, right and C to E) with the following significance levels: (*P<0.05, **P<0.01,***P<0.001). ns, not significant.

品牌：Liposoma

產地：荷蘭

貨號：CP-005-005

規(guī)格：5ml+5ml

品名：Clodronateliposomes & Control Liposomes

代理：靶點科技

部位：肺臟

細胞：肺泡巨噬細胞（AM）

劑量：70ul

方式：滴鼻（intratracheal administration）

時間點：建模前48h

題目：Lung-innervating nociceptor sensory neurons promote pneumonic sepsis during carbapenem-resistant Klebsiella pneumoniae lung infection